This blog has information on Alternatives to Conventional Medicine, natural medicine, homeopathy vancouver, Homeoprophylaxis, Homeopathic Immunizations, and holistic health practitioners
Friday, June 21, 2013
Friday, April 19, 2013
breast cancer cells
1Integrative Medicine Program, 2Department of Molecular Pathology, 3Department of Melanoma Medical Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, TX, USA;
4P. Banerji Homeopathic Research Foundation, Kolkata, India Received May 28, 2009; Accepted July 23, 2009 DOI: 10.3892/ijo_00000512
Abstract. The use of ultra-diluted natural products in the management of disease and treatment of cancer has generated a lot of interest and controversy. We conducted an in vitro study to determine if products prescribed by a clinic in India have any effect on breast cancer cell lines. We studied four ultra-diluted remedies (Carcinosin, Phytolacca, Conium and Thuja) against two human breast adenocarcinoma cell lines (MCF-7 and MDA-MB-231) and a cell line derived from immortalized normal human mammary epithelial cells (HMLE). The remedies exerted preferential cytotoxic effects against the two breast cancer cell lines, causing cell cycle delay/arrest and apoptosis. These effects were accompanied by altered expression of the cell cycle regulatory proteins, including downregulation of phosphorylated Rb and upregulation of the CDK inhibitor p27, which were likely responsible for the cell cycle delay/arrest as well as induction of the apoptotic cascade that manifested in the activation of caspase 7 and cleavage of PARP in the treated cells. The findings demonstrate biological activity of these natural products when presented at ultra-diluted doses. Further in- depth studies with additional cell lines and animal models are warranted to explore the clinical applicability of these agents.
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Tuesday, March 26, 2013
Inhibition of basophil activation by histamine: a sensitive and reproducible model for the study of the biological activity of high dilutions
Volume 98, Issue 4, October 2009, Pages 186-197
Special Issue: Biological models of homeopathy Part 1
doi:10.1016/j.homp.2009.09.009 | How to Cite or Link Using DOI
Copyright © 2009 The Faculty of Homeopathy Published by Elsevier Ltd. Cited By in Scopus (5)
Permissions & Reprints Inhibition of basophil activation by histamine: a sensitive and reproducible model for the study of the biological activity of high dilutions J. Sainte-Laudy1, Corresponding Author Contact Information, E-mail The Corresponding Author and Ph Belon2 1CHU, Limoges 87042, France 2CRDT, 45 cours A Briand, 69300 Caluire, France
Received 8 July 2009;
revised 21 September 2009;
accepted 23 September 2009.
Available online 27 November 2009. Background At the beginning of this series of experiments we were looking for a model based on the use of purified commercially available compounds based on a fully described and accepted pharmacological model to study of the biological effect of high dilutions. Negative feedback induced by histamine, a major pro-inflammatory mediator, on basophils and mast cells activation via an H2 receptor me these criteria. The simplest way of measuring basophil activation in the early 1980's was the human basophil activation test (HBDT).
Objectives Our major goal was first to study the biological effect of centesimal histamine dilutions beyond the Avogadro limit, on the staining properties of human basophils activated by an allergen extract initially house dust mite, then an anti-IgE and N-formyl-Met-Leu-Phe (fMLP). Technical development over the 25 years of our work led us to replace the manual basophil counting by flow cytometry. The main advantages were automation and observer independence. Using this latter protocol our aim was to confirm the existence of this phenomenon and to check its specificity by testing, under the same conditions, inactive analogues of histamine and histamine antagonists. More recently, we developed an animal model (mouse basophils) to study the effect of histamine on histamine release.
Methods and results For the HBDT model basophils were obtained by sedimentation of human blood taken on EDTA and stained with Alcian blue. Results were expressed in percentage activation. Histamine dilutions tested were freshly prepared in the lab by successive centesimal dilutions and vortexing. Water controls were prepared in the same way. For the flow cytometric protocol basophils were first labeled by an anti-IgE FITC (basophil marker) and an anti-CD63 (basophil activation marker). Results were expressed in percentage of CD63 positive basophils. Another flow cytometric protocol has been developed more recently, based on basophil labeling by anti-IgE FITC (fluorescein isothiocyanate) and anti-CD203 PE (another human basophil activation marker). Results were expressed in mean fluorescence intensity of the CD203c positive population (MFI-CD203c) and an activation index calculated by an algorithm. For the mouse basophil model, histamine was measured spectrofluorimetrically. The main results obtained over 28 years of work was the demonstration of a reproducible inhibition of human basophil activation by high dilutions of histamine, the effect peaks in the range of 15?17CH. The effect was not significant when histamine was replaced by histidine (a histamine precursor) or cimetidine (histamine H2 receptor antagonist) was added to the incubation medium. These results were confirmed by flow cytometry. Using the latter technique, we also showed that 4-Methyl histamine (H2 agonist) induced a similar effect, in contrast to 1-Methyl histamine, an inactive histamine metabolite. Using the mouse model, we showed that histamine high dilutions, in the same range of dilutions, inhibited histamine release.
Conclusions Successively, using different models to study of human and murine basophil activation, we demonstrated that high dilutions of histamine, in the range of 15?17CH induce a reproducible biological effect. This phenomenon has been confirmed by a multi-center study using the HBDT model and by at least three independent laboratories by flow cytometry. The specificity of the observed effect was confirmed, versus the water controls at the same dilution level by the absence of biological activity of inactive compounds such as histidine and 1-Methyl histamine and by the reversibility of this effect in the presence of a histamine receptor H2 antagonist. Keywords: Human basophil; Mouse basophil; High dilutions; Homoeopathy; Histamine; Flow cytometry; Histamine release; IL4 release
Article Outline Introduction Human basophil pharmacology Preparation of high dilutions Analysis of human basophil activation by their metachromatic properties Analysis of human basophil activation by flow cytometry Relationships between results and hypotheses related to the mode of action of high dilutions
Hypothesis derived from biological experiments
Hypotheses derived from physical experiments Conclusions Acknowledgements Figure 1. Human basophil stained by Alcian blue among unstained Polymorphonuclear cells. Figure 2. Effect of histamine dilutions from 10−10 to 10−120 M, showing recurrent inhibition of activation. Basophil activation triggered by house dust mite extract. *p
Figure 3. Set up of flow cytometric protocol based on the double anti-IgE and anti-CD63 staining. Figure 4. Effect of histamine dilutions from 10CH (10) to 20CH (20) on anti-IgE induced human basophil activation versus the water controls diluted in the same conditions. Compared to water control 16C, the effect of histamine 16C was significant (p
Figure 5. Inhibition of anti-IgE induced human basophil activation by histamine 15CH and 16CH. Results expressed in %CD63 ± SD versus the positive and negative controls prepared with water 16C. Figure 6. Comparison of the effect of high dilutions of histamine and histidine on anti-IgE induced human basophil activation (mean of 13 experiments in triplicates). Results expressed in% CD63 ± SD versus positive and negative controls prepared in water 16C. NS = not significant. Figure 7. Antagonist effect of lithium 10 μg/ml on inhibition of anti-IgE induced human basophil activation by histamine 15CH and 16CH. Results expressed in %CD63 ± SD, NS = not significant. Figure 8. Set up of flow cytometric protocol for the analysis of CD203c up-regulation on activated human basophil membrane. Figure 9. Inhibition of fMLP-induced basophil activation by histamine 16CH and histamine 2CH. Results expressed versus negative and positive controls prepared in water 16 C and expressed in MFI-CD203c ± SD. Negative controls set at 10. Statistical significance calculated on the raw data by Wilcoxon rank test. Figure 10. Effect of 1, 3, 4-methyl histamine 16CH and histamine 16CH on fMLP-induced basophil activation. Basophil activation expressed in MFI-CD203c ± SEM, mean of 10 experiments in triplicates. Negative controls (not shown) were set at 10 to compare the different experiments performed on different blood donors. NS = not significant. Figure 11. Effect of histamine 15CH and 16CH on histamine production by mouse total bone marrow cells stimulated by IgE versus water controls tested at the same dilution level. View Within Article
Table 1. Published results related to the inhibition of basophil activation by histamine dilutions
View table in article ND = not done, NS = not significant.
1 significance calculated versus the related water control.
2 Not significant versus the whole series of water controls. View Within Article Corresponding Author Contact InformationCorrespondence: J Sainte-Laudy, Laboratoire d'Immunologie, Hôpital universitaire Dupuytren, 2, avenue Martin-Luther-King, 87042 Limoges, Cedex, France.
Monday, March 11, 2013
Japanese Encephalitis
Decreased Intensity of Japanese Encephalitis Virus Infection in Chick Chorioallantoic Membrane Under Influence of Ultradiluted Belladonna Extract 1Bhaswati Bandyopadhyay, 2Satadal Das, 1Milan Sengupta, 3Chandan Saha, 4Kartick Chandra Das, 4Debabrata Sarkar and 5Chaturbhuj Nayak 1Department of Microbiology, Virology Unit, School of Tropical Medicine, Kolkata-700073, India 2Department of Pathology and Microbiology, D.N. De H. Medical College, West Bengal University of Health Sciences, Kolkata-700046, India 3Department of Clinical and Experimental Pharmacology, School of Tropical Medicine, Kolkata-700073, India 4Drug Proving Research Centre, CCRH, Government of India, Kolkata-700 046, India 5Department of AYUSH, Ministry of Health, CCRH, Government of India, JLN Anudandhan Bhawan, 61-65 Intitutional Area, Janakpuri, New Delhi 110058
Abstract: Problem statement: No specific antiviral therapy is currently available despite an emergence and resurgence of Japanese encephalitis in South-East Asian Countries. There are only few recent studies, which were aimed to treat Japanese encephalitis with newer drugs. There is thus a real need for study on antiviral agents that can reduce the toll of death and neurological sequelae resulting from infection with this virus. Approach: Optimum dilution of the JE virus was determined which could produce significant number of pocks on Chorioallantoic Membrane (CAM). Then ultradiluted belladonna preparations were used to see their inhibitory action on JE virus infection in CAM. Results: Ultradiluted belladonna showed significantly decreased pock count in CAM in comparison to JE virus control. Conclusion: Ultradiluted belladonna could inhibit JE virus infection in CAM, which may be mediated through glycosidase inhibitory role of calystegines present in belladonna.
Key words: Japanese Encephalitis (JE), Chorioallantoic Membrane (CAM), pock, belladonna
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Friday, March 8, 2013
Homeopathy on Vitality Link
http://www.vitalitylink.com/blog/practitioners-stories/practitioner-elena-cecchetto-homeopath/
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Monday, March 4, 2013
Mastitis
Saturday, February 23, 2013
New Book for a good Doctor-Patient Relationship
"...Fear of the possibility of loss makes us hold more tightly onto what we possess. A rigid hostility to any change underlies a fragile sense of security. 'Better the devil you know than the one you don't.' Love on the other hand, is light and expansive. It's an expression of our trust in the ultimate goodness of the world, and of our sense of all thing sharing their place in the universe. Love makes life feel like a continuum, and a soul's journey in the body feel as part of a greater whole..." ..." it appears that direct patient involvement int he process of healing, established through their on interpretation of the meaning of their symptomatology, as well as a clear visual formulation of their intention to potentiate a healing action, is a 'prescription' that will yield the best results. The doctor's role is to help educate and support the process..."
..." If we redefine the 'placebo effect,' or the 'positivo effect,' then we have no preconceived negatives to fall back on. We are, in essesnce, setting the stage for specific goals and outcomes by providing daily routines to reinforce them. We also provide inspiration and hope, but with direction, goals and specificity. What if the 'aligned and committed effect' was in operation while the patients received effective treatment free from negative side effects?..."
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Friday, February 22, 2013
Homeopathy Editorial
More from National Post
French Nobel Medicine Prize winner Luc Montagnier is pictured on October 6, 2008 at the presidential palace in Abidjan. Montagnier dedicated his award to AIDS sufferers and predicted results on a "therapeutic vaccine" for the pandemic within four years. Montagnier and Francoise Barre-Sinoussi, who shared the Nobel prize, discovered the human immunodeficiency virus (HIV) that causes AIDS by destroying immune cells, one of the scourges of modern times.
Issouf Sanogo / AFP / Getty ImagesFrench Nobel Medicine Prize winner Luc Montagnier is pictured on October 6, 2008 at the presidential palace in Abidjan. Montagnier dedicated his award to AIDS sufferers and predicted results on a "therapeutic vaccine" for the pandemic within four years. Montagnier and Francoise Barre-Sinoussi, who shared the Nobel prize, discovered the human immunodeficiency virus (HIV) that causes AIDS by destroying immune cells, one of the scourges of modern times. Every now and then, someone in the media falsely claims that there is little or no evidence supporting the practice of homeopathy. They either cherry-pick their references ? as Timothy Caulfield did in Tuesday?s National Post ? or lump homeopathy in with less well-established and non-standardized practices, such as ?faith healing? or ?energy healing,? implying it has less significance than it does. What most Canadians aren?t aware of is homeopathy?s true stature and importance worldwide ? and how fast it is gaining acceptance and both in Canada and abroad. Homeopathy is so well trusted that 300 million patients in more than 80 nations use it. In countries such as the U.K., Brazil, parts of India, Mexico and Cuba, homeopathy is integrated into the health system and covered by public health insurance. In Europe, three out of four people are familiar with it. In Cuba, mass dosing of preventive homeopathic medicines is now used routinely by the public health system for epidemic control. One of the world?s most popular over-the-counter flu medicines ? Oscillococcinum ? is a homeopathic remedy. Homeopathy is arguably the fastest-growing system of medicine in the world. The Associated Chambers of Commerce and Industry of India reported in March 2011 that India?s market for homeopathy was worth approximately $5.35-billion, and growing by about 30% annually. In the U.S., where the FDA recognizes the 1938 American Homeopathic Pharmacopoeia as the official reference guiding the manufacture of homeopathic medicines, their use has increased fivefold since 1990. Homeopathy is now a regulated health profession in Ontario, and homeopathic medicines are classified by Health Canada. A massive study showing that homeopathy is more cost-effective than any other forms of medicine, traditional or alternative, was commissioned by the government of Switzerland and published in 2011. Perhaps other governments struggling with spiralling health-care costs should take heed. There are, in fact, many promising studies on homeopathy, across a broad number of fields. Of the meta-analyses ? studies measuring the number and results of existing studies ? that have been published, the majority show findings promising enough to recommend further research in the field. The largest single study of homeopathy ever published was conducted under the auspices of the Cuban Ministry of Health in 2007. The populations of the three provinces of Cuba most threatened by the hurricane-triggered disease leptospirosis ? a total of 2.3 million people ? were all given two doses of a preventative homeopathic medicine in advance of the time of worst danger. The result: ?The homeoprophylactic approach was associated with a large reduction of disease incidence and control of the epidemic.? There is no magic or witchcraft in homeopathy. Anyone of any (or no) religious or spiritual tradition can practise it with training, and the patient does not have to believe in it for it to work (else it wouldn?t affect infants, animals and microbes). In homeopathy, positive results require the use of standard and repeatable procedures based on consistent principles, which are the core of the curricula of homeopathic colleges. Homeopathy?s big stumbling block to acceptance is that its medicines are diluted so much that people outside of the field can?t understand how they can possibly have an effect. There are, however many scientists who do have that expertise. So many, that there is an entire journal devoted to the field, the International Journal of High Dilution Research. And they seem to be getting intriguingly close to providing definitive answers. Opponents of homeopathy claim that homeopathic medicines are ?just plain water? with no medicinal properties. But increasing numbers of scientific findings are making it harder to maintain such as stance. One study has found that solutions prepared in the traditional homeopathic way ? through repeated dilutions by mechanical shaking ? have properties unlike plain water, with elements of the dissolved material. Another study suggests the solutions have an affect on living cells in vitro. Yet another study shows that solutions can be distinguished from each other, using the right equipment to determine their contents. And emerging research suggests that homeopathic solutions actually contain nanoparticles of the original dissolved material. It?s not quacks or junk scientists researching high dilutions. Dr. Luc Montagnier, Nobel laureate and co-discoverer of the human immunodeficiency virus, presented at a national American homeopathic conference last year, discussing his work on the ability of DNA in high dilutions to emit electromagnetic waves. The question is: How do we tackle a phenomenon that defies our notion of reality and yet clearly shows promise? The scientific process knows how: Test it, investigate it, measure it with ever-more-sophisticated instruments ? while always staying open to the possibility that even widely-held notions of reality may be proven wrong. Rigorous open-mindedness ? being prepared to give up your preconceptions when evidence contradicts them ? is the core of science. And that?s how the rest of us need to approach this issue, if we are serious about using every option available to alleviate human illness in our health-care system. National Post Karen Wehrstein is the executive director of the Canadian Consumers Centre for Homeopathy (homeocentre.ca), an organization formed in 2011 to educate the public about homeopathy and advocate for freedom of choice in health care.
Thursday, February 21, 2013
Nature and technology
~From what I'm reading; "The Dance of Molecules" by Ted Sargent.
Your Holistic Team, ~Access~
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CEASE Therapy case examples for AUTISM
http://homeoinfo.org/
Homeopathy Works
Your Holistic Team, ~Access~
Web: http://www.AccessNaturalHealing.com/
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Tuesday, February 19, 2013
Testimonial
Your Holistic Team, ~Access~
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Friday, February 1, 2013
CEASE Therapy for Autism
Your Holistic Team, ~Access~
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Tuesday, January 29, 2013
Homeopathy Offers Hope
Every now and then, someone in the media falsely claims that there is little or no evidence supporting the practice of homeopathy. They either cherry-pick their references — as Timothy Caulfield did in Tuesday’s National Post — or lump homeopathy in with less well-established and non-standardized practices, such as “faith healing” or “energy healing,” implying it has less significance than it does. What most Canadians aren’t aware of is homeopathy’s true stature and importance worldwide — and how fast it is gaining acceptance and both in Canada and abroad. Homeopathy is so well trusted that 300 million patients in more than 80 nations use it. In countries such as the U.K., Brazil, parts of India, Mexico and Cuba, homeopathy is integrated into the health system and covered by public health insurance. In Europe, three out of four people are familiar with it. In Cuba, mass dosing of preventive homeopathic medicines is now used routinely by the public health system for epidemic control. One of the world’s most popular over-the-counter flu medicines — Oscillococcinum — is a homeopathic remedy. Homeopathy is arguably the fastest-growing system of medicine in the world. The Associated Chambers of Commerce and Industry of India reported in March 2011 that India’s market for homeopathy was worth approximately $5.35-billion, and growing by about 30% annually. In the U.S., where the FDA recognizes the 1938 American Homeopathic Pharmacopoeia as the official reference guiding the manufacture of homeopathic medicines, their use has increased fivefold since 1990. Homeopathy is now a regulated health profession in Ontario, and homeopathic medicines are classified by Health Canada. A massive study showing that homeopathy is more cost-effective than any other forms of medicine, traditional or alternative, was commissioned by the government of Switzerland and published in 2011. Perhaps other governments struggling with spiralling health-care costs should take heed. There are, in fact, many promising studies on homeopathy, across a broad number of fields. Of the meta-analyses — studies measuring the number and results of existing studies — that have been published, the majority show findings promising enough to recommend further research in the field. The largest single study of homeopathy ever published was conducted under the auspices of the Cuban Ministry of Health in 2007. The populations of the three provinces of Cuba most threatened by the hurricane-triggered disease leptospirosis — a total of 2.3 million people — were all given two doses of a preventative homeopathic medicine in advance of the time of worst danger. The result: “The homeoprophylactic approach was associated with a large reduction of disease incidence and control of the epidemic.” There is no magic or witchcraft in homeopathy. Anyone of any (or no) religious or spiritual tradition can practise it with training, and the patient does not have to believe in it for it to work (else it wouldn’t affect infants, animals and microbes). In homeopathy, positive results require the use of standard and repeatable procedures based on consistent principles, which are the core of the curricula of homeopathic colleges. Homeopathy’s big stumbling block to acceptance is that its medicines are diluted so much that people outside of the field can’t understand how they can possibly have an effect. There are, however many scientists who do have that expertise. So many, that there is an entire journal devoted to the field, the International Journal of High Dilution Research. And they seem to be getting intriguingly close to providing definitive answers. Opponents of homeopathy claim that homeopathic medicines are “just plain water” with no medicinal properties. But increasing numbers of scientific findings are making it harder to maintain such as stance. One study has found that solutions prepared in the traditional homeopathic way — through repeated dilutions by mechanical shaking — have properties unlike plain water, with elements of the dissolved material. Another study suggests the solutions have an affect on living cells in vitro. Yet another study shows that solutions can be distinguished from each other, using the right equipment to determine their contents. And emerging research suggests that homeopathic solutions actually contain nanoparticles of the original dissolved material. It’s not quacks or junk scientists researching high dilutions. Dr. Luc Montagnier, Nobel laureate and co-discoverer of the human immunodeficiency virus, presented at a national American homeopathic conference last year, discussing his work on the ability of DNA in high dilutions to emit electromagnetic waves. The question is: How do we tackle a phenomenon that defies our notion of reality and yet clearly shows promise? The scientific process knows how: Test it, investigate it, measure it with ever-more-sophisticated instruments — while always staying open to the possibility that even widely-held notions of reality may be proven wrong. Rigorous open-mindedness — being prepared to give up your preconceptions when evidence contradicts them — is the core of science. And that’s how the rest of us need to approach this issue, if we are serious about using every option available to alleviate human illness in our health-care system. National Post, January 28, 2013 Karen Wehrstein is the executive director of the Canadian Consumers Centre for Homeopathy (homeocentre.ca), an organization formed in 2011 to educate the public about homeopathy and advocate for freedom of choice in health care.
Your Holistic Team, ~Access~
Web: http://www.AccessNaturalHealing.com/
Email: info@accessnaturalhealing.com
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“The information contained in this message is for educational purposes and constitutes a response to a private request for information only and does not constitute a solicitation for services and makes no claim or promise that any product or service that may cure any condition or ailment,”
Thursday, January 24, 2013
Stomach Flu Easy Remedies
infections in the stomach and intestines. Medically, such an infected
condition is termed as 'Viral Gastroenteritis'. Swallowing any of the
different viruses, including rotavirus, adenovirus or the norwalk virus,
might initiate the problem. The infection is also communicable and
sometimes develops from physical contact with an infected person and even
spreads through infected utensils, food and drink. Once these viruses
enter your digestive tract, they damage the linings of the various organs,
thereby leading to stomach cramps, dizziness, vomiting, fever, loss of
appetite, chills and muscle ache. Though the symptoms sound quite
hazardous, various home remedies can easily help you to get rid of the
problem. Check out some easy-to-follow cure and treatment of stomach
viruses here. Home Remedy for Stomach Virus
First, do not consume anything for a couple of hours and allow the
stomach to clear up. Sip small amounts of water, apple juice or weak
tea.
After fasting for a day, introduce bland and soft food to your diet.
Eat boiled rice, apples, boiled lentil broth, clear soup, etc.
Do not consume milk, orange juice, greasy foods, tomato-based food and
dairy products for a few days.
Abstain from drinking alcohol, coffee and carbonated drinks until you
recover. Start drinking lots of water and electrolyte fluids.
Drink a solution of grapefruit seed extract (5 drops) and a glass of
water. It has useful antimicrobial properties and is also an effective
disinfectant.
Drink a concoction of freshly squeezed lemon juice and water, 6-7
times a day. You can also add flat soda to the mixture.
Drinking a glass of red or white wine after dinner is another
effective solution.
Drinking cranberry juice can do wonders to dispel stomach viruses.
Consume fresh cranberry juice, without any additives.
Stomach virus can lead to a highly fatigued body. So, do not forget to
take long hours of rest. Plus the Homeopathic Remedies:
Arsenicum
Veratrum Album
Nux Vomica
Ipecac
Podophylum
Wednesday, January 16, 2013
Strychnos nux-vomica extract and its ultra-high dilution reduce voluntary ethanol intake in rats.
Strychnos nux-vomica extract and its ultra-high dilution reduce voluntary ethanol intake in rats.
Authors
Sukul NC, Ghosh S, Sinhababu SP, et al.
Institution
Department of Zoology, Visva-Bharati University, Santiniketan, West Bengal, India. nirmal@vbharat.ernet.in
Source
J Altern Complement Med 2001 Apr; 7(2) :187-93.
Abstract
To see whether Strychnos nux-vomica extract (mother tincture [MT]), its potency Nux 30c, and its principal alkaloid, strychnine, could reduce voluntary ethanol intake in rats. To analyze the solution structure of Nux MT, Nux 30c, 90% ethanol, and ethanol 30c by means of electronic (ES) and nuclear nuclear magnetic resonance (NMR) spectra.Potentially alcoholic rats were first given 20% ethanol and then kept on a two-choice bottle, one with 20% ethanol and another with tap water. These rats were given the following oral treatments for 15 days: group 1, control; group 2, strychnine at 0.36 mg/kg per day; group 3, ethanolic extract of S. nux-vomica seeds (Nux MT) at 3.6 mg/kg per day; and group 4, Nux 30c at 0.05 mL/d per rat. Nux 30c was prepared by successive dilution of Nux MT and 90% ethanol (1:100) and sonication at 20 kHz for 30 seconds in 30 steps.Both Nux MT and Nux 30c significantly reduced ethanol intake and increased water intake in rats. ES of two dilutions of Nux MT and Nux 30c showed intersections at more than one point suggesting existence of molecular complexes. ES of Nux MT in CCl4 showed a red shift when 90% ethanol was added indicating molecular complexation and charge transfer interaction between ethanol and Nux compounds. NMR spectra of Nux MT, 90% ethanol, ethanol 30c, and Nux 30c indicated a change in solution structure of the medium (90% ethanol) of Nux 30c.Nux MT and Nux 30c could reduce ethanol intake in rats. The altered solution structure of Nux 30c is thought to mimic Nux MT and produce ethanol aversion in rats.
Mesh
Alcohol Deterrents
Alcoholism
Animals
Disease Models, Animal
Ethanol
Feeding Behavior
Homeopathy
Plant Extracts
Plants, Medicinal
Rats
Strychnine
Language
eng
Pub Type(s)
Journal Article
PubMed ID
11327524